The rise of bacteria resistant to antibiotics has become a significant issue in contemporary microbiology and public health. Escherichia coli (E. coli) is frequently examined due to its clinical importance and its ability to quickly adapt to antibiotics. This research examines the cultivation of ampicillin-resistant E. coli on agar plates with ampicillin to study bacterial growth, colony appearance, and resistance characteristics. The bacterial strain was grown using standard microbiological methods, and its growth was assessed under controlled laboratory conditions. The results show that resistant colonies successfully grew on selective media, indicating the presence of resistance genes that enable survival in the presence of ampicillin.