The physiochemical tests were conducted using Vitex negundo and Eclipta prostrata. To fully grasp the physiological state and therapeutic qualities of plants, seasonal fluctuation is the most crucial factor to consider. The various environmental factors have been observed to cause fluctuations in the concentration of inorganic elements, photosynthetic pigments (such as chlorophyll a, b, and carotenoids), photosynthetic enzymes (such as RuBPCase), oxidative enzymes (such as IAA oxidase), nitrogen metabolism enzymes (such as Nitrate reductase and Nitrite reductase), organic constituents (such as nitrogen, polyphenol, and proline), and inorganic elemental concentration in V. negundo and E. prostrata. Carbohydrates, proteins, glycosides, saponins, flavonoids, steroids, tannins, and phenolic substances were identified in the screening of early phytochemical studies and characterisation of several extracts of V. negundo and E. prostrata. In addition to lending credence to the traditional usage of E. prostrata, the results of the current study may aid in the fight against inflammation and allergies.