This research explores the diversity of monocotyledonous seedlings in the forests of Dakshin Dinajpur District, West Bengal, focusing on their role in forest regeneration and ecosystem stability. The study involved a detailed analysis of various monocot families, including Poaceae, Arecaceae, and Orchidaceae, highlighting their distribution, abundance, and ecological significance. The findings underscore the importance of conserving these seedlings as they play a crucial role in maintaining the biodiversity and health of the forest ecosystem. The research also provides insights into the environmental factors influencing the growth and distribution of these monocotyledonous species, contributing to a better understanding of the region's flora and the need for targeted conservation efforts.