abstract

Trichoderma species are well-known for their biocontrol potential against various plant pathogens, making them promising candidates for sustainable agriculture. In this study, we conducted a comparative analysis of Trichoderma isolates focusing on molecular profiling and their antagonistic activity against key agricultural foliar pathogens. “Trichoderma isolates were collected from different ecological niches, including soil, plant roots, and decaying organic matter. Molecular characterization involved PCR amplification of internal transcribed spacer (ITS) regions, sequencing, and phylogenetic analysis to identify species composition and genetic diversity. Antagonistic activity assays were conducted using dual-culture methods against prominent foliar plant pathogens, including Fusarium spp., Alternaria spp., and Botrytis spp. The inhibition zone diameter was measured to quantify biocontrol efficacy. Our findings revealed a diverse species composition and genetic diversity among Trichoderma isolates, with notable variations in antagonistic activity against different pathogens. Certain isolates exhibited superior biocontrol efficacy, demonstrating the potential for tailored biocontrol strategies. This comparative analysis provides valuable insights into harnessing Trichoderma-based biocontrol agents for integrated disease management in agriculture, emphasizing the importance of genetic screening and strain selection for optimal biocontrol performance.